Dionex  DNA還內Pac PA100 寡核苷酸色譜柱

Thermo Scientific? Dionex? DNAPac? PA10自下0 寡核苷酸柱的特點是采用強陰離子交換樹脂，從而可對(duì地妹)合成(chéng)型和經(jīng)過(guò)修飾的寡核苷酸進(玩來jìn)行高分辨率分析和純化。

描述

• 13μm、薄膜型、無孔、聚合物樹脂，并帶有與季胺官能(西地néng)團鍵合的 MicroBeads?

• 可對(duì)寡核苷酸進(jìn)行 n、n-1 分離

• 解析次級結構的寡核苷酸

• 與溶劑、高 pH 和高溫相容

• 分析基于硫代磷酸酯的臨床樣(yàng)品

• 可輕松升級

Dionex  DNAPac 可房PA100 寡核苷酸色譜柱

訂貨信息：

For n, n-1 Resolution of Si兵大ngle-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolutio制購n anion-exchange column that離間 provides single-base resolution. It訊遠 is stable under den做場aturing conditions還長, rugged, reliable, and ca少畫n be readily scaled廠師 up. The DNAPac PA100 is a 13 μm 件工pellicular, nonporous p紙習olymeric resin with bound煙黃 quaternary amine-functionali在化zed MicroBeads^?. The rapid mass-海事transport characteristic科嗎s of this resin resul關車t in very high-光音resolution oligonucleotide 從制separations.

• Capable of n, n-1 r兒一esolution for oligonucleotides

• Resolves oligonucleotides with s生爸econdary structures慢朋

• Compatible with solvent, 坐商high pH, and high temperatures從舞

• Analyzes phosphor區北othioate-based clinical samples

• Provides easy s書路cale-up

The MicroBead res白紅in used in DNAPac columns provides裡村 high-resolution separations to e拿銀nsure maximum pea什問k purity, and makes linear sc書姐ale-up of your separation easy. F國厭or more complex separations, the po學用lymeric properties of身算 the DNAPac PA100妹坐 allows great fl場術exibility to select ideal co懂通nditions, including high pH, high麗黃 temperature, or chaotropic age習開nts.

Synthetic oligonucleotides can be窗得 screened for product文間ion yield and failure sequences公購 using the DNAPac PA1城低00. Unit-base resolution of身她 synthetic oligonucleotides to 60 ba快熱ses and beyond has been demonst知錯rated. The DNAPac PA100 can eve綠工n be operated under denaturin們筆g conditions. Either high temperatur工場e (up to 90 °慢美;C) or high-pH eluen票些ts (up to pH 12.5) can b有這e used to eliminate hydro東西gen bonding, allowing 黃森resolution of problem sequences書刀 such as self-complementa話雨ry sequences or poly路離-G stretches.

Separates RNA / D身有ouble-Stranded DNA

The DNAPac PA100 is ideally suite黃費d for the purification and analysis of 房信synthetic RNA. Failure se是體quences are easily separated from the 影那full-length product. Double事文-stranded DNA, such計樹 as plasmids, or restriction fragmen身道ts are also separat站器ed.

Analytical separations on the 4 mm d短吧iameter column can be scaled directly 秒市to larger diameter columns so prepa為森rative methods can be 湖紙conveniently develop南門ed using small samples. The loading cap水紙acity of the DNAPac PA100 (4 × 25討鄉0 mm) column is from 1 to 100的體 μg. Scaling the flow ra子好te and sample size up for th紅費e 9 × 250 mm and 22 &ti開身mes; 250 mm column yields essentially 兵樹identical chromatography.山個