多潘立酮與氟哌利多的分離-Zodiac C18 色譜柱要相

更新時(shí)間：2021-07-20 &nbs道匠p; 點擊次數：1373次

多潘立酮與氟哌利多的分離-Zodiac C18服去色譜柱

Column : C18 100mm*4.6mm, 3µm
Flow Rate: 1.5ml/min
Wave Length : 280nm
Temperature: 25°知光C
Runtime : 20 minutes

Preparation of 流動相:國她

1.) : 流動相A
道草Dissolve 5.0g of Ammonium Acetate in 也信1000ml of HPLC Gr器飛ade Water

2.) 流動相 : B
&nb時校sp; Methanol HPLC Grade

3.) Gradient Program :

Time (min)	流動相-A(%V/V)	流動相- B(%V/V)
0-10	70	30
10-15	0	100
15-15.01	70	30
15.01-20	70	30

4.) Diluent : N,N Dimethylformami頻吧de
Domperidone Reference Solution (a) : (關好0.10 and 0.15 mg/ml會白) Weight accurately about 10mg 好一of Domperidone CRS and 15會懂mg of Dro他房peridol CRS into a 100ml Volumetr北日ic flask dissolve and dilute 嗎著to volume with Dil購拍uent and mix Inject 10µl of Dom兒志peridone Reference Soluti些了on (a) in duplicate and calculate Res窗線olution between Do拿相mperidone and Droperidol.

Acceptance Criteria:

Resolution between D也自omperidone and Dr間女opridol should not be less than 2.0

Column Name : Zodiac 月輛C18 Column

Compound Name : Droperidol, Domperidon東朋e

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