高效液相色譜法分離阿昔洛韋和氫化混合相場物-Primesep 100

高效液相色譜法分離阿昔洛韋和氫做木化混合物

Compounds of significantly厭喝 different polarity are easy to resolve個會 on reverse phase HP通農LC columns. In this situation,多舊 a gradient elution is u大見sually required to obtain a鄉要 reasonable retention time for all co秒公mpounds. However the 綠場gradient is not always desirable得話 especially for high throughput m地些ode of operation.

• Extra work would be required t但花o prepare two different solvents知相 for theL流動相 phase.
• Run time is long.
• Additional time is required for col自女umn equilibration prior to ea山間ch injection.

A mixture of acyclovir and h工照ydrocortisone is a such exa朋市mple. Often they are both 內物constituted in topi快高cal creams used to tr到爸eat cold sores/fever bliste森外rs. Acyclovir is a polar 一黑molecule (logP = – 1.2) while h線科ydrocortisone (logP = 綠放~1.7) is a hydr拿睡ophobic compound. Their separati厭土on on a reverse p事窗hase 150 mm, 5ìm column would ta商喝ke 10+ minutes. The same separation務錢, but much faster, can be o們朋btained on a Mixed-Mode (日器Primesep 100) column with simple isoc慢著ratic mode of elution.兵嗎 For LC-MS compatible conditions the 低理sulfuric acid can be subst笑但ituted by TFA or ammonium fo拍北rmate.

Column Name : Primesep 100 Columns

Compound Name : Hydrocortisone, Acyclovir